Photo by Jerry Harb
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...
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...
encoding="utf-8"
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?>
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<rss
...
version="2.0">
...
<channel>
...
<title>pubmed:
...
anthony
...
a
...
james</title>
...
<link>https://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Search&db=PubMed&term=
...
anthony%20a%20james</link>
...
<description>NCBI:
...
db=pubmed;
...
Term=anthony
...
a
...
james</description>
...
<language>en-us</language>
...
<docs>http://blogs.law.harvard.edu/tech/
...
rss</docs>
...
<ttl>1440</ttl>
...
<image>
<title>NCBI pubmed</title>
...
<url>https://www.ncbi.nlm.nih.gov/entrez/query/static/gifs/iconsml.
...
gif</url>
...
<link>https://www.ncbi.nlm.nih.gov/sites/
...
entrez</link>
...
<description>PubMed
...
comprises
...
more
...
than
...
millions
...
of
...
citations
...
for
...
biomedical
...
literature
...
from
...
MEDLINE,
...
life
...
science
...
journals,
...
and
...
online
...
books.
...
Citations
...
may
...
include
...
links
...
to
...
full-text
...
content
...
from
...
PubMed
...
Central
...
and
...
publisher
...
web
...
sites.</description>
...
</image>
...
<item>
<title>Transgenic Clustered Regularly Interspaced Short Palindromic Repeat/Cas9-Mediated
...
Viral
...
Gene
...
Targeting
...
for
...
Antiviral
...
Therapy
...
of
...
Bombyx
...
mori
...
Nucleopolyhedrovirus.</title>
...
<link>https://www.ncbi.nlm.nih.gov/pubmed/28122981?dopt=
...
Abstract</link>
...
<description>
<![CDATA[<table
...
border="0"
...
width="100%"><tr><td
...
align="left"><a
...
href="http://jvi.asm.org/cgi/pmidlookup?view=long&
...
pmid=28122981"><img
...
src="//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:-
...
highwire.stanford.edu-icons-externalservices-pubmed-standard-jvi_full_free.gif"
...
border="0"/></a>
...
<a
...
href="http://jvi.asm.org/cgi/pmidlookup?view=long&
...
pmid=28122981"><img
...
src="//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:
...
highwire.stanford.edu-icons-externalservices-pubmed-standard-jvi_final.gif"
...
border="0"/></a>
...
<a
...
href="https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/28122981/"><img
...
src="//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:
...
-www.ncbi.nlm.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif"
...
border="0"/></a>
...
</td><td
...
align="right"><a
...
href="https://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&
...
cmd=Link&
...
LinkName=pubmed_pubmed&
...
from_uid=28122981">Related
...
Articles</a></td></tr></table>
...
<p><b>Transgenic Clustered Regularly Interspaced Short Palindromic Repeat/Cas9-Mediated
...
Viral
...
Gene
...
Targeting
...
for
...
Antiviral
...
Therapy
...
of
...
Bombyx
...
mori
...
Nucleopolyhedrovirus.</b></p>
...
<p>J Virol.
...
2017
...
Apr
...
15;91(8):</p>
...
<p>Authors:
...
Chen
...
S,
...
Hou
...
C,
...
Bi
...
H,
...
Wang
...
Y,
...
Xu
...
J,
...
Li
...
M,
...
James
...
AA,
...
Huang
...
Y,
...
Tan
...
A</p>
...
<p>Abstract<br/>
...
We developed a novel antiviral strategy by combining transposon-based
...
transgenesis
...
and
...
the
...
clustered
...
regularly
...
interspaced
...
short
...
palindromic
...
repeats
...
(CRISPR)/CRISPR-associated
...
9
...
(Cas9)
...
system
...
for
...
the
...
direct
...
cleavage
...
of
...
Bombyx
...
mori
...
nucleopolyhedrovirus
...
(BmNPV)
...
genome
...
DNA
...
to
...
promote
...
virus
...
clearance
...
in
...
silkworms.
...
We
...
demonstrate
...
that
...
transgenic
...
silkworms
...
constitutively
...
expressing
...
Cas9
...
and
...
guide
...
RNAs
...
targeting
...
the
...
BmNPV
...
immediate
...
early-1
...
(ie-1)
...
and
...
me53
...
genes
...
effectively
...
induce
...
target-specific
...
cleavage
...
and
...
subsequent
...
mutagenesis,
...
especially
...
large
...
(∼7-kbp)
...
segment
...
deletions
...
in
...
BmNPV
...
genomes,
...
and
...
thus
...
exhibit
...
robust
...
suppression
...
of
...
BmNPV
...
proliferation.
...
Transgenic
...
animals
...
exhibited
...
higher
...
and
...
inheritable
...
resistance
...
to
...
BmNPV
...
infection
...
than
...
wild-type
...
animals.
...
Our
...
approach
...
will
...
not
...
only
...
contribute
...
to
...
modern
...
sericulture
...
but
...
also
...
shed
...
light
...
on
...
future
...
antiviral
...
therapy.IMPORTANCE
...
Pathogen
...
genome
...
targeting
...
has
...
shown
...
its
...
potential
...
in
...
antiviral
...
research.
...
However,
...
transgenic
...
CRISPR/Cas9
...
system-mediated
...
viral
...
genome
...
targeting
...
has
...
not
...
been
...
reported
...
as
...
an
...
antiviral
...
strategy
...
in
...
a
...
natural
...
animal
...
host
...
of
...
a
...
virus.
...
Our
...
data
...
provide
...
an
...
effective
...
approach
...
against
...
BmNPV
...
infection
...
in
...
a
...
real-world
...
biological
...
system
...
and
...
demonstrate
...
the
...
potential
...
of
...
transgenic
...
CRISPR/Cas9
...
systems
...
in
...
antiviral
...
research
...
in
...
other
...
species.<br/>
...
</p><p>PMID:
...
28122981
...
...
...
...
...
...
</p>
...
]]></description>
...
<author>
...
Chen
...
S,
...
Hou
...
C,
...
Bi
...
H,
...
Wang
...
Y,
...
Xu
...
J,
...
Li
...
M,
...
James
...
AA,
...
Huang
...
Y,
...
Tan
...
A</author>
...
<category>J
...
Virol</category>
...
<guid
...
isPermaLink="false">PubMed:28122981</guid>
...
</item>
...
<item>
<title>Bombyx mori P-element
...
Somatic
...
Inhibitor
...
(BmPSI)
...
Is
...
a
...
Key
...
Auxiliary
...
Factor
...
for
...
Silkworm
...
Male
...
Sex
...
Determination.</title>
...
<link>https://www.ncbi.nlm.nih.gov/pubmed/28103247?dopt=
...
Abstract</link>
...
<description>
<![CDATA[<table
...
border="0"
...
width="100%"><tr><td
...
align="left"><a
...
href="http://dx.plos.org/10.1371/journal.pgen.1006576"><img
...
src="//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:
...
-journals.plos.org-plosgenetics-resource-img-external-pgen_120x30.png"
...
border="0"/></a>
...
<a
...
href="https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/28103247/"><img
...
src="//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:
...
-www.ncbi.nlm.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif"
...
border="0"/></a>
...
</td><td
...
align="right"><a
...
href="https://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&
...
cmd=Link&
...
LinkName=pubmed_pubmed&
...
from_uid=28103247">Related
...
Articles</a></td></tr></table>
...
<p><b>Bombyx mori P-element
...
Somatic
...
Inhibitor
...
(BmPSI)
...
Is
...
a
...
Key
...
Auxiliary
...
Factor
...
for
...
Silkworm
...
Male
...
Sex
...
Determination.</b></p>
...
<p>PLoS Genet.
...
2017
...
Jan;13(1):e1006576</p>
...
<p>Authors:
...
Xu
...
J,
...
Chen
...
S,
...
Zeng
...
B,
...
James
...
AA,
...
Tan
...
A,
...
Huang
...
Y</p>
...
<p>Abstract<br/>
...
Manipulation of sex determination pathways in insects provides the basis for a wide spectrum of strategies to benefit agriculture and public health. Furthermore, insects display a remarkable diversity in the genetic pathways that lead to sex differentiation. The silkworm, Bombyx mori, has been cultivated by humans as a beneficial insect for over two millennia, and more recently as a model system for studying lepidopteran genetics and development. Previous studies have identified the B. mori Fem piRNA as the primary female determining factor and BmMasc as its downstream target, while the genetic scenario for male sex determination was still unclear. In the current study, we exploite the transgenic CRISPR/Cas9 system to generate a comprehensive set of knockout mutations in genes BmSxl, Bmtra2, BmImp, BmImpM, BmPSI and BmMasc, to investigate their roles in silkworm sex determination. Absence of Bmtra2 results in the complete depletion of Bmdsx transcripts, which is the conserved downstream factor in the sex determination pathway, and induces embryonic lethality. Loss of BmImp or BmImpM function does not affect the sexual differentiation. Mutations in BmPSI and BmMasc genes affect the splicing of Bmdsx and the female reproductive apparatus appeared in the male external genital. Intriguingly, we identify that BmPSI regulates expression of BmMasc, BmImpM and Bmdsx, supporting the conclusion that it acts as a key auxiliary factor in silkworm male sex determination.<br/>
</p><p>PMID: 28103247 PubMed - indexed for MEDLINE</p>
]]></description>
<author> Xu J, Chen S, Zeng B, James AA, Tan A, Huang Y</author>
<category>PLoS Genet</category>
<guid isPermaLink="false">PubMed:28103247</guid>
...
</item>
...
<item>
<title>Sexually dimorphic traits in the silkworm, Bombyx mori, are regulated by doublesex.</title>
...
<link>https://www.ncbi.nlm.nih.gov/pubmed/27867075?dopt=
...
Abstract</link>
<description>
<![CDATA[<table
...
border="0"
...
width="100%"><tr><td
...
align="left"><a
...
href="https://linkinghub.elsevier.com/retrieve/pii/S0965-1748(16)30192-8"><img
...
src="//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif"
...
border="0"/></a>
...
</td><td
...
align="right"><a
...
href="https://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&
...
cmd=Link&
...
LinkName=pubmed_pubmed&
...
from_uid=27867075">Related
...
Articles</a></td></tr></table>
...
<p><b>Sexually dimorphic traits in the silkworm, Bombyx mori, are regulated by doublesex.</b></p>
...
<p>Insect Biochem Mol Biol. 2017 Jan;80:42-51</p>
...
<p>Authors:
...
Xu
...
J,
...
Zhan
...
S,
...
Chen
...
S,
...
Zeng
...
B,
...
Li
...
Z,
...
James
...
AA,
...
Tan
...
A,
...
Huang
...
Y</p>
...
<p>Abstract<br/>
...
The DM domain genes,
...
doublesex
...
(dsx)
...
in
...
insects,
...
or
...
their
...
structural
...
homologs,
...
male
...
abnormal
...
3
...
(mab-3)
...
in
...
nematodes
...
and
...
Dmrt1
...
(doublesex
...
and
...
mab-3-related
...
transcription
...
factor
...
1)
...
in
...
mammals,
...
are
...
downstream
...
regulators
...
of
...
the
...
sex
...
determination
...
pathway
...
that
...
control
...
sexually
...
dimorphic
...
development.
...
Despite
...
the
...
functional
...
importance
...
of
...
dsx
...
and
...
its
...
potential
...
applications
...
in
...
sterile
...
insect
...
technologies
...
(SITs),
...
the
...
mechanisms
...
by
...
which
...
it
...
controls
...
sexually
...
dimorphic
...
traits
...
and
...
the
...
subsequent
...
developmental
...
gene
...
networks
...
in
...
insects
...
are
...
poorly
...
understood.
...
Phylogenetic
...
analyses
...
indicate
...
that
...
insect
...
dsx
...
genes
...
have
...
sex-specific
...
alternative
...
splicing
...
isoforms,
...
whereas
...
other
...
taxa
...
do
...
not.
...
We
...
exploited
...
genome
...
editing
...
and
...
transgenesis
...
technologies
...
to
...
induce
...
mutations
...
in
...
either
...
the
...
male-specific
...
isoform
...
(dsx(M))
...
or
...
common
...
region
...
(dsx(C))
...
of
...
dsx
...
in
...
the
...
somatic
...
tissues
...
of
...
the
...
lepidopteran
...
model
...
insect
...
Bombyx
...
mori.
...
Disruptions
...
of
...
gene
...
function
...
produced
...
either
...
male-specific
...
sexually-dimorphic
...
defects
...
or
...
intersexual
...
phenotypes;
...
these
...
results
...
differ
...
from
...
those
...
observed
...
in
...
other
...
insects,
...
including
...
Drosophila
...
melanogaster.
...
Our
...
data
...
provide
...
insights
...
into
...
the
...
divergence
...
of
...
the
...
insect
...
sex
...
determination
...
pathways
...
related
...
to
...
the
...
most
...
conserved
...
downstream
...
component
...
dsx.<br/>
...
</p><p>PMID:
...
27867075
...
...
...
...
...
</p>
...
]]></description>
...
<author>
...
Xu
...
J,
...
Zhan
...
S,
...
Chen
...
S,
...
Zeng
...
B,
...
Li
...
Z,
...
James
...
AA,
...
Tan
...
A,
...
Huang
...
Y</author>
...
<category>Insect
...
Biochem
...
Mol
...
Biol</category>
...
<guid
...
isPermaLink="false">PubMed:27867075</guid>
...
</item>
...
<item>
<title>rAed a 4:
...
A
...
New
...
67-kDa
...
Aedes
...
aegypti
...
Mosquito
...
Salivary
...
Allergen
...
for
...
the
...
Diagnosis
...
of
...
Mosquito
...
Allergy.</title>
...
<link>https://www.ncbi.nlm.nih.gov/pubmed/27603527?dopt=
...
Abstract</link>
...
<description>
<![CDATA[<table
...
border="0"
...
width="100%"><tr><td
...
align="left"><a
...
href="https://www.karger.com?DOI=10.1159/000448587"><img
...
src="//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--misc.karger.com-LinkOutIcons-sk_nlm_ft.gif"
...
border="0"/></a>
...
</td><td
...
align="right"><a
...
href="https://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&
...
cmd=Link&
...
LinkName=pubmed_pubmed&
...
from_uid=27603527">Related
...
Articles</a></td></tr></table>
...
<p><b>rAed a 4:
...
A
...
New
...
67-kDa
...
Aedes
...
aegypti
...
Mosquito
...
Salivary
...
Allergen
...
for
...
the
...
Diagnosis
...
of
...
Mosquito
...
Allergy.</b></p>
...
<p>Int Arch Allergy Immunol. 2016;170(3):206-10</p>
...
<p>Authors:
...
Peng
...
Z,
...
Caihe
...
L,
...
Beckett
...
AN,
...
Guan
...
Q,
...
James
...
AA,
...
Simons
...
FE</p>
...
<p>Abstract<br/>
...
BACKGROUND:
...
Accurate
...
diagnosis
...
of
...
mosquito
...
allergy
...
has
...
been
...
hampered
...
by
...
the
...
laborious
...
task
...
of
...
obtaining
...
mosquito
...
salivary
...
allergens.
...
We
...
have
...
previously
...
studied
...
3
...
recombinant
...
(r)
...
Aedes
...
aegypti
...
mosquito
...
salivary
...
allergens:
...
rAed
...
a
...
1,
...
rAed
...
a
...
2
...
and
...
rAed
...
a
...
3.
...
Here,
...
we
...
report
...
the
...
expression,
...
purification,
...
identification
...
and
...
evaluation
...
of
...
rAed
...
a
...
4,
...
a
...
67-kDa
...
α-glucosidase.<br/>
...
METHODS:
...
rAed
...
a
...
4
...
was
...
expressed
...
using
...
a
...
baculovirus/insect
...
cell
...
system,
...
purified
...
by
...
a
...
combination
...
of
...
anion-
...
and
...
cation-exchange
...
chromatography,
...
and
...
identified
...
by
...
immunoblotting.
...
A.
...
aegypti
...
saliva
...
extract
...
was
...
prepared
...
in
...
our
...
laboratory.
...
An
...
indirect
...
enzyme-linked
...
immunosorbent
...
assay
...
(ELISA)
...
was
...
developed
...
to
...
measure
...
rAed
...
a
...
4-specific
...
immunoglobulin
...
E
...
(IgE)
...
and
...
IgG
...
antibodies
...
in
...
sera
...
from
...
13
...
individuals
...
with
...
a
...
positive
...
mosquito-bite
...
test
...
from
...
a
...
laboratory-reared
...
mosquito.
...
Sera
...
from
...
18
...
individuals
...
with
...
a
...
negative
...
bite
...
test
...
served
...
as
...
controls.<br/>
...
RESULTS:
...
Purified
...
rAed
...
a
...
4
...
bound
...
to
...
the
...
IgE
...
in
...
mosquito-allergic
...
sera,
...
as
...
detected
...
by
...
ELISA
...
and
...
immunoblotting.
...
The
...
binding
...
of
...
rAed
...
a
...
4
...
to
...
IgE
...
could
...
be
...
inhibited
...
in
...
a
...
dose-dependent
...
manner
...
by
...
the
...
addition
...
of
...
an
...
A.
...
aegypti
...
extract.
...
Mosquito-allergic
...
individuals
...
had
...
significantly
...
higher
...
mean
...
levels
...
of
...
rAed
...
a
...
4-specific
...
IgE
...
and
...
IgG
...
than
...
controls.
...
Using
...
the
...
mean
...
of
...
the
...
controls
...
±
...
2
...
SD
...
as
...
a
...
cut-off
...
level,
...
46%
...
of
...
the
...
13
...
allergic
...
individuals
...
had
...
a
...
positive
...
IgE,
...
while
...
none
...
of
...
the
...
controls
...
was
...
positive
...
(p
...
< 0.001).<br/>
...
CONCLUSIONS:
...
Aed
...
a
...
4
...
is
...
a
...
major
...
allergen
...
in
...
mosquito
...
saliva.
...
Its
...
recombinant
...
form
...
has
...
the
...
hydrolase
...
function
...
and
...
can
...
be
...
used
...
for
...
the
...
diagnosis
...
of
...
mosquito
...
allergy.<br/>
...
</p><p>PMID:
...
27603527
...
...
...
...
...
...
</p>
...
]]></description>
...
<author>
...
Peng
...
Z,
...
Caihe
...
L,
...
Beckett
...
AN,
...
Guan
...
Q,
...
James
...
AA,
...
Simons
...
FE</author>
...
<category>Int
...
Arch
...
Allergy
...
Immunol</category>
...
<guid
...
isPermaLink="false">PubMed:27603527</guid>
...
</item>
...
<item>
<title>Functional analysis of Orco and odorant receptors in odor recognition in Aedes albopictus.</title>
...
<link>https://www.ncbi.nlm.nih.gov/pubmed/27350348?dopt=
...
Abstract</link>
...
<description>
<![CDATA[<table
...
border="0"
...
width="100%"><tr><td
...
align="left"><a
...
href="https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-016-1644-9"><img
...
src="//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:
...
-www.biomedcentral.com-graphics-pubmed-BioMedCentral_free_1.png"
...
border="0"/></a>
...
<a
...
href="https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/27350348/"><img
...
src="//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:
...
-www.ncbi.nlm.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif"
...
border="0"/></a>
...
</td><td
...
align="right"><a
...
href="https://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&
...
cmd=Link&
...
LinkName=pubmed_pubmed&
...
from_uid=27350348">Related
...
Articles</a></td></tr></
...
table>
<p><b>Functional analysis of Orco and odorant receptors in odor recognition in Aedes albopictus.</b></p>
...
<p>Parasit Vectors.
...
2016
...
06
...
27;9(1):363</p>
...
<p>Authors:
...
Liu
...
H,
...
Liu
...
T,
...
Xie
...
L,
...
Wang
...
X,
...
Deng
...
Y,
...
Chen
...
CH,
...
James
...
AA,
...
Chen
...
XG</p>
...
<p>Abstract<br/>
...
BACKGROUND:
...
Aedes
...
albopictus
...
is
...
a
...
globally
...
invasive
...
mosquito
...
and
...
a
...
major
...
vector
...
of
...
arboviruses,
...
including
...
dengue,
...
Zika
...
and
...
Chikungunya.
...
Olfactory-related
...
behaviors,
...
particularly
...
host-seeking,
...
offer
...
opportunities
...
to
...
disrupt
...
the
...
disease-transmission
...
process.
...
A
...
better
...
understanding
...
of
...
odorant
...
receptors
...
(ORs)
...
may
...
assist
...
in
...
explaining
...
host
...
selection
...
and
...
location,
...
and
...
contribute
...
to
...
novel
...
strategy
...
of
...
vector
...
control.<br/>
...
METHODS:
...
Based
...
on
...
previous
...
prediction
...
of
...
158
...
putative
...
odorant
...
receptors
...
by
...
Ae.
...
albopictus
...
genome
...
analysis,
...
29
...
AalORs
...
were
...
selected
...
for
...
tissue-specific
...
expression
...
profiles
...
in
...
the
...
present
...
study.
...
AalOrco
...
(AalOR7),
...
AalOR10
...
and
...
AalOR88,
...
highly
...
expressed
...
in
...
female
...
olfactory
...
tissues,
...
were
...
chosen
...
for
...
further
...
structure
...
predictions
...
as
...
well
...
as
...
functional
...
validation
...
including
...
calcium
...
imaging
...
assay
...
in
...
human
...
embryonic
...
kidney
...
(HEK293)
...
cells
...
and
...
RNA
...
interference
...
assay
...
in
...
Ae.
...
albopictus.
...
We
...
also
...
conducted
...
electrophysiological
...
and
...
behavioral
...
assays
...
in
...
mosquitoes
...
after
...
RNA
...
interference
...
of
...
the
...
three
...
genes
...
to
...
determine
...
their
...
roles
...
in
...
host-seeking.<br/>
...
RESULTS:
...
The
...
results
...
support
...
previous
...
conclusions
...
that
...
individual
...
conventional
...
(ORXs)
...
and
...
Orco
...
can
...
form
...
heteromeric
...
complexes
...
to
...
recognize
...
odorants
...
and
...
respond
...
to
...
components
...
of
...
human
...
volatiles
...
in
...
HEK293
...
cells.
...
The
...
reduction
...
of
...
AalOrco
...
transcript
...
levels
...
led
...
to
...
a
...
significant
...
decrease
...
in
...
host-seeking
...
and
...
confusion
...
in
...
host
...
preference.
...
In
...
contrast,
...
AalOR10
...
and
...
AalOR88
...
knockdown
...
mosquitoes
...
showed
...
no
...
significant
...
behavioral
...
differences
...
compared
...
with
...
controls.
...
The
...
functions
...
of
...
conventional
...
ORs
...
at
...
least
...
AalOR10
...
and
...
AalOR88
...
are
...
abolished
...
with
...
inhibited
...
expression
...
of
...
the
...
Orco
...
gene
...
orthologs,
...
along
...
with
...
the
...
concomitant
...
relevant
...
olfactory
...
behavior.<br/>
...
CONCLUSIONS:
...
Combining
...
structural
...
and
...
functional
...
data,
...
we
...
conclude
...
that
...
the
...
product
...
of
...
the
...
Orco
...
gene
...
in
...
this
...
mosquito
...
is
...
crucial
...
for
...
transmitting
...
olfactory
...
signaling
...
and
...
conventional
...
ORs
...
contribute
...
directly
...
to
...
odorant
...
recognition.
...
Our
...
results
...
provide
...
insight
...
into
...
the
...
linkage
...
between
...
odorant
...
receptors
...
and
...
host-seeking
...
in
...
this
...
important
...
vector
...
species.<br/>
...
</p><p>PMID:
...
27350348
...
...
...
...
...
...
</p>
...
]]></description>
...
<author>
...
Liu
...
H,
...
Liu
...
T,
...
Xie
...
L,
...
Wang
...
X,
...
Deng
...
Y,
...
Chen
...
CH,
...
James
...
AA,
...
Chen
...
XG</author>
...
<category>Parasit
...
Vectors</category>
...
<guid
...
isPermaLink="false">PubMed:27350348</guid>
...
</item>
...
<item>
<title>CRISPR/Cas9
...
mediated
...
knockout
...
of
...
the
...
abdominal-A
...
homeotic
...
gene
...
in
...
the
...
global
...
pest,
...
diamondback
...
moth
...
(Plutella
...
xylostella).</title>
...
<link>https://www.ncbi.nlm.nih.gov/pubmed/27318252?dopt=
...
Abstract</link>
...
<description>
<![CDATA[<table
...
border="0"
...
width="100%"><tr><td
...
align="left"><a
...
href="https://linkinghub.elsevier.com/retrieve/pii/S0965-1748(16)30081-9"><img
...
src="//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif"
...
border="0"/></a>
...
</td><td
...
align="right"><a
...
href="https://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&
...
cmd=Link&
...
LinkName=pubmed_pubmed&
...
from_uid=27318252">Related
...
Articles</a></td></tr></table>
...
<p><b>CRISPR/Cas9
...
mediated
...
knockout
...
of
...
the
...
abdominal-A
...
homeotic
...
gene
...
in
...
the
...
global
...
pest,
...
diamondback
...
moth
...
(Plutella
...
xylostella).</b></p>
...
<p>Insect Biochem Mol Biol. 2016 Aug;75:98-106</
...
p>
<p>Authors:
...
Huang
...
Y,
...
Chen
...
Y,
...
Zeng
...
B,
...
Wang
...
Y,
...
James
...
AA,
...
Gurr
...
GM,
...
Yang
...
G,
...
Lin
...
X,
...
Huang
...
Y,
...
You
...
M</p>
...
<p>Abstract<br/>
...
The diamondback moth,
...
Plutella
...
xylostella
...
(L.),
...
is
...
a
...
worldwide
...
agricultural
...
pest
...
that
...
has
...
developed
...
resistance
...
to
...
multiple
...
classes
...
of
...
insecticides.
...
Genetics-based
...
approaches
...
show
...
promise
...
as
...
alternative
...
pest
...
management
...
approaches
...
but
...
require
...
functional
...
studies
...
to
...
identify
...
suitable
...
gene
...
targets.
...
Here
...
we
...
use
...
the
...
CRISPR/Cas9
...
system
...
to
...
target
...
a
...
gene,
...
abdominal-A,
...
which
...
has
...
an
...
important
...
role
...
in
...
determining
...
the
...
identity
...
and
...
functionality
...
of
...
abdominal
...
segments.
...
We
...
report
...
that
...
P.
...
xylostella
...
abdominal-A
...
(Pxabd-A)
...
has
...
two
...
structurally-similar
...
splice
...
isoforms
...
(A
...
and
...
B)
...
that
...
differ
...
only
...
in
...
the
...
length
...
of
...
exon
...
II,
...
with
...
15
...
additional
...
nucleotides
...
in
...
isoform
...
A.
...
Pxabd-A
...
transcripts
...
were
...
detected
...
in
...
all
...
developmental
...
stages,
...
and
...
particularly
...
in
...
pupae
...
and
...
adults.
...
CRISPR/Cas9-based
...
mutagenesis
...
of
...
Pxabd-A
...
exon
...
I
...
produced
...
91%
...
chimeric
...
mutants
...
following
...
injection
...
of
...
448
...
eggs.
...
Phenotypes
...
with
...
abnormal
...
prolegs
...
and
...
malformed
...
segments
...
were
...
visible
...
in
...
hatched
...
larvae
...
and
...
unhatched
...
embryos,
...
and
...
various
...
defects
...
were
...
inherited
...
by
...
the
...
next
...
generation
...
(G1).
...
Genotyping
...
of
...
mutants
...
demonstrated
...
several
...
mutations
...
at
...
the
...
Pxabd-A
...
genomic
...
locus.
...
The
...
results
...
indicate
...
that
...
a
...
series
...
of
...
insertions
...
and
...
deletions
...
were
...
induced
...
in
...
the
...
Pxabd-A
...
locus,
...
not
...
only
...
in
...
G0
...
survivors
...
but
...
also
...
in
...
G1
...
individuals,
...
and
...
this
...
provides
...
a
...
foundation
...
for
...
genome
...
editing.
...
Our
...
study
...
demonstrates
...
the
...
utility
...
of
...
the
...
CRISPR/Cas9
...
system
...
for
...
targeting
...
genes
...
in
...
an
...
agricultural
...
pest
...
and
...
therefore
...
provides
...
a
...
foundation
...
the
...
development
...
of
...
novel
...
pest
...
management
...
tools.
...
<br/>
...
</p><p>PMID:
...
27318252
...
...
...
...
...
</p>
...
]]></description>
...
<author>
...
Huang
...
Y,
...
Chen
...
Y,
...
Zeng
...
B,
...
Wang
...
Y,
...
James
...
AA,
...
Gurr
...
GM,
...
Yang
...
G,
...
Lin
...
X,
...
Huang
...
Y,
...
You
...
M</author>
...
<category>Insect
...
Biochem
...
Mol
...
Biol</category>
...
<guid
...
isPermaLink="false">PubMed:27318252</guid>
...
</item>
...
<item>
<title>Endogenously-expressed
...
NH2-terminus
...
of
...
circumsporozoite
...
protein
...
interferes
...
with
...
sporozoite
...
invasion
...
of
...
mosquito
...
salivary
...
glands.</title>
...
<link>https://www.ncbi.nlm.nih.gov/pubmed/26964736?dopt=
...
Abstract</link>
...
<description>
<![CDATA[<table
...
border="0"
...
width="100%"><tr><td
...
align="left"><a
...
href="https://malariajournal.biomedcentral.com/articles/10.1186/s12936-016-1207-8"><img
...
src="//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:-
...
www.biomedcentral.com-graphics-pubmed-BioMedCentral_free_1.png"
...
border="0"/></a>
...
<a
...
href="https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/26964736/"><img
...
src="//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:
...
-www.ncbi.nlm.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif"
...
border="0"/></a>
...
</td><td
...
align="right"><a
...
href="https://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&
...
cmd=Link&
...
LinkName=pubmed_pubmed&
...
from_uid=26964736">Related
...
Articles</a></td></tr></table>
...
<p><b>Endogenously-expressed
...
NH2-terminus
...
of
...
circumsporozoite
...
protein
...
interferes
...
with
...
sporozoite
...
invasion
...
of
...
mosquito
...
salivary
...
glands.</b></p>
...
<p>Malar J.
...
2016
...
Mar
...
10;15:153</p>
...
<p>Authors:
...
Kojin
...
BB,
...
Costa-da-Silva
...
AL,
...
Maciel
...
C,
...
Henriques
...
DA,
...
Carvalho
...
DO,
...
Martin
...
K,
...
Marinotti
...
O,
...
James
...
AA,
...
Bonaldo
...
MC,
...
Capurro
...
ML</p>
...
<p>Abstract<br/>
...
BACKGROUND:
...
The
...
circumsporozoite
...
protein
...
is
...
the
...
most
...
abundant
...
polypeptide
...
expressed
...
by
...
sporozoites,
...
the
...
malaria
...
parasite
...
stage
...
capable
...
of
...
infecting
...
humans.
...
Sporozoite
...
invasion
...
of
...
mosquito
...
salivary
...
glands
...
prior
...
to
...
transmission
...
is
...
likely
...
mediated
...
by
...
a
...
receptor/ligand-like
...
interaction
...
of
...
the
...
parasites
...
with
...
the
...
target
...
tissues,
...
and
...
the
...
amino
...
(NH2)-terminal
...
portion
...
of
...
CSP
...
is
...
involved
...
in
...
this
...
interaction
...
but
...
not
...
the
...
TSR
...
region
...
on
...
the
...
carboxyl
...
(C)-terminus.
...
Peptides
...
based
...
on
...
the
...
NH2-terminal
...
domain
...
could
...
compete
...
with
...
the
...
parasites
...
for
...
the
...
salivary
...
gland
...
receptors
...
and
...
thus
...
inhibit
...
penetration.<br/>
...
METHODS:
...
Peptides
...
based
...
on
...
the
...
NH2-terminus
...
and
...
TSR
...
domains
...
of
...
the
...
CSP
...
from
...
avian
...
or
...
human
...
malaria
...
parasites,
...
Plasmodium
...
gallinaceum
...
and
...
Plasmodium
...
falciparum,
...
respectively,
...
were
...
expressed
...
endogenously
...
in
...
mosquito
...
haemolymph
...
using
...
a
...
transient
...
(Sindbis
...
virus-mediated)
...
or
...
stable
...
(piggyBac-mediated
...
transgenesis)
...
system.<br/>
...
RESULTS:
...
Transient
...
endogenous
...
expression
...
of
...
partial
...
NH2-terminus
...
peptide
...
from
...
P.
...
falciparum
...
CSP
...
in
...
P.
...
gallinaceum-infected
...
Aedes
...
aegypti
...
resulted
...
in
...
a
...
reduced
...
number
...
of
...
sporozoites
...
in
...
the
...
salivary
...
glands.
...
When
...
a
...
transgenic
...
approach
...
was
...
used
...
to
...
express
...
a
...
partial
...
CSP
...
NH2-terminal
...
domain
...
from
...
P.
...
gallinaceum
...
the
...
number
...
of
...
sporozoites
...
in
...
the
...
salivary
...
glands
...
did
...
not
...
show
...
a
...
difference
...
when
...
compared
...
to
...
controls.
...
However,
...
a
...
significant
...
difference
...
could
...
be
...
observed
...
when
...
mosquitoes
...
with
...
a
...
lower
...
infection
...
were
...
analysed.
...
The
...
same
...
result
...
could
...
not
...
be
...
observed
...
with
...
mosquitoes
...
endogenously
...
expressing
...
peptides
...
based
...
on
...
the
...
TSR
...
domain
...
from
...
either
...
P.
...
gallinaceum
...
or
...
P.
...
falciparum.<br/>
...
CONCLUSION:
...
These
...
results
...
support
...
the
...
conclusion
...
that
...
CSP
...
partial
...
NH2-terminal
...
domain
...
can
...
be
...
endogenously
...
expressed
...
to
...
promote
...
a
...
competition
...
for
...
the
...
receptor
...
used
...
by
...
sporozoites
...
to
...
invade
...
salivary
...
glands,
...
and
...
they
...
could
...
be
...
used
...
to
...
block
...
this
...
interaction
...
and
...
reduce
...
parasite
...
transmission.
...
The
...
same
...
effect
...
cannot
...
be
...
obtained
...
with
...
peptides
...
based
...
on
...
the
...
TSR
...
domain.<br/
...
>
</p><p>PMID:
...
26964736
...
...
...
...
...
...
</p>
...
]]></description>
...
<author>
...
Kojin
...
BB,
...
Costa-da-Silva
...
AL,
...
Maciel
...
C,
...
Henriques
...
DA,
...
Carvalho
...
DO,
...
Martin
...
K,
...
Marinotti
...
O,
...
James
...
AA,
...
Bonaldo
...
MC,
...
Capurro
...
ML</author>
...
<category>Malar
...
J</category>
...
<guid
...
isPermaLink="false">PubMed:26964736</guid>
...
</item>
...
<item>
<title>Highly efficient Cas9-mediated
...
gene
...
drive
...
for
...
population
...
modification
...
of
...
the
...
malaria
...
vector
...
mosquito
...
Anopheles
...
stephensi.</title>
...
<link>https://www.ncbi.nlm.nih.gov/pubmed/26598698?dopt=
...
Abstract</link>
...
<description>
<![CDATA[<table
...
border="0"
...
width="100%"><tr><td
...
align="left"><a
...
href="http://www.pnas.org/cgi/pmidlookup?view=long&
...
pmid=26598698"><img
...
src="//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:
...
-highwire.stanford.edu-icons-externalservices-pubmed-custom-pnas_full_free.gif"
...
border="0"/></a>
...
<a
...
href="http://www.pnas.org/cgi/pmidlookup?view=long&
...
pmid=26598698"><img
...
src="//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:
...
highwire.stanford.edu-icons-externalservices-pubmed-custom-pnas_full_free.gif"
...
border="0"/></a>
...
<a
...
href="https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/26598698/"><img
...
src="//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:
...
-www.ncbi.nlm.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif"
...
border="0"/></a>
...
</td><td
...
align="right"><a
...
href="https://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&
...
cmd=Link&
...
LinkName=pubmed_pubmed&
...
from_uid=26598698">Related
...
Articles</a></td></tr></table>
...
<p><b>Highly efficient Cas9-mediated
...
gene
...
drive
...
for
...
population
...
modification
...
of
...
the
...
malaria
...
vector
...
mosquito
...
Anopheles
...
stephensi.</b></p>
...
<p>Proc Natl Acad Sci U S A. 2015 Dec 08;112(49):E6736-43</p>
...
<p>Authors:
...
Gantz
...
VM,
...
Jasinskiene
...
N,
...
Tatarenkova
...
O,
...
Fazekas
...
A,
...
Macias
...
VM,
...
Bier
...
E,
...
James
...
AA</p>
...
<p>Abstract<br/>
...
Genetic engineering technologies can be used both to create transgenic mosquitoes carrying antipathogen effector genes targeting human malaria parasites and to generate gene-drive systems capable of introgressing the genes throughout wild vector populations. We developed a highly effective autonomous Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated
...
protein
...
9
...
(Cas9)-mediated
...
gene-drive
...
system
...
in
...
the
...
Asian
...
malaria
...
vector
...
Anopheles
...
stephensi,
...
adapted
...
from
...
the
...
mutagenic
...
chain
...
reaction
...
(MCR).
...
This
...
specific
...
system
...
results
...
in
...
progeny
...
of
...
males
...
and
...
females
...
derived
...
from
...
transgenic
...
males
...
exhibiting
...
a
...
high
...
frequency
...
of
...
germ-line
...
gene
...
conversion
...
consistent
...
with
...
homology-directed
...
repair
...
(HDR).
...
This
...
system
...
copies
...
an
...
∼
...
17-kb
...
construct
...
from
...
its
...
site
...
of
...
insertion
...
to
...
its
...
homologous
...
chromosome
...
in
...
a
...
faithful,
...
site-specific
...
manner.
...
Dual
...
anti-Plasmodium
...
falciparum
...
effector
...
genes,
...
a
...
marker
...
gene,
...
and
...
the
...
autonomous
...
gene-drive
...
components
...
are
...
introgressed
...
into
...
∼
...
99.5%
...
of
...
the
...
progeny
...
following
...
outcrosses
...
of
...
transgenic
...
lines
...
to
...
wild-type
...
mosquitoes.
...
The
...
effector
...
genes
...
remain
...
transcriptionally
...
inducible
...
upon
...
blood
...
feeding.
...
In
...
contrast
...
to
...
the
...
efficient
...
conversion
...
in
...
individuals
...
expressing
...
Cas9
...
only
...
in
...
the
...
germ
...
line,
...
males
...
and
...
females
...
derived
...
from
...
transgenic
...
females,
...
which
...
are
...
expected
...
to
...
have
...
drive
...
component
...
molecules
...
in
...
the
...
egg,
...
produce
...
progeny
...
with
...
a
...
high
...
frequency
...
of
...
mutations
...
in
...
the
...
targeted
...
genome
...
sequence,
...
resulting
...
in
...
near-Mendelian
...
inheritance
...
ratios
...
of
...
the
...
transgene.
...
Such
...
mutant
...
alleles
...
result
...
presumably
...
from
...
nonhomologous
...
end-joining
...
(NHEJ)
...
events
...
before
...
the
...
segregation
...
of
...
somatic
...
and
...
germ-line
...
lineages
...
early
...
in
...
development.
...
These
...
data
...
support
...
the
...
design
...
of
...
this
...
system
...
to
...
be
...
active
...
strictly
...
within
...
the
...
germ
...
line.
...
Strains
...
based
...
on
...
this
...
technology
...
could
...
sustain
...
control
...
and
...
elimination
...
as
...
part
...
of
...
the
...
malaria
...
eradication
...
agenda.<br/>
...
</p><p>PMID:
...
26598698
...
...
...
...
...
...
</p>
...
]]></description>
...
<author>
...
Gantz
...
VM,
...
Jasinskiene
...
N,
...
Tatarenkova
...
O,
...
Fazekas
...
A,
...
Macias
...
VM,
...
Bier
...
E,
...
James
...
AA</author>
...
<category>Proc
...
Natl
...
Acad
...
Sci
...
U
...
S
...
A</category>
...
<guid
...
isPermaLink="false">PubMed:26598698</guid>
...
</item>
...
<item>
<title>Genome sequence of the Asian Tiger mosquito, Aedes albopictus, reveals insights into its biology, genetics, and evolution.</title>
<link>https://www.ncbi.nlm.nih.gov/pubmed/26483478?dopt=
...
Abstract</link>
...
<description>
<![CDATA[<table
...
border="0"
...
width="100%"><tr><td
...
align="left"><a
...
href="http://www.pnas.org/cgi/pmidlookup?view=long&
...
pmid=26483478"><img
...
src="//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:-
...
highwire.stanford.edu-icons-externalservices-pubmed-custom-pnas_full_free.gif"
...
border="0"/></a>
...
<a
...
href="http://www.pnas.org/cgi/pmidlookup?view=long&
...
pmid=26483478"><img
...
src="//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:
...
highwire.stanford.edu-icons-externalservices-pubmed-custom-pnas_full_free.gif"
...
border="0"/></a>
...
<a
...
href="https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/26483478/"><img
...
src="//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:
...
-www.ncbi.nlm.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif"
...
border="0"/></a>
...
</td><td
...
align="right"><a
...
href="https://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&
...
cmd=Link&
...
LinkName=pubmed_pubmed&
...
from_uid=26483478">Related
...
Articles</a></td></tr></table>
...
<p><b>Genome sequence of the Asian Tiger mosquito, Aedes albopictus, reveals insights into its biology, genetics, and evolution.</b></p>
<p>Proc Natl Acad Sci U S A. 2015 Nov 03;112(44):E5907-15</p>
...
<p>Authors:
...
Chen
...
XG,
...
Jiang
...
X,
...
Gu
...
J,
...
Xu
...
M,
...
Wu
...
Y,
...
Deng
...
Y,
...
Zhang
...
C,
...
Bonizzoni
...
M,
...
Dermauw
...
W,
...
Vontas
...
J,
...
Armbruster
...
P,
...
Huang
...
X,
...
Yang
...
Y,
...
Zhang
...
H,
...
He
...
W,
...
Peng
...
H,
...
Liu
...
Y,
...
Wu
...
K,
...
Chen
...
J,
...
Lirakis
...
M,
...
Topalis
...
P,
...
Van
...
Leeuwen
...
T,
...
Hall
...
AB,
...
Jiang
...
X,
...
Thorpe
...
C,
...
Mueller
...
RL,
...
Sun
...
C,
...
Waterhouse
...
RM,
...
Yan
...
G,
...
Tu
...
ZJ,
...
Fang
...
X,
...
James
...
AA</p>
...
<p>Abstract<br/>
...
The Asian tiger mosquito,
...
Aedes
...
albopictus,
...
is
...
a
...
highly
...
successful
...
invasive
...
species
...
that
...
transmits
...
a
...
number
...
of
...
human
...
viral
...
diseases,
...
including
...
dengue
...
and
...
Chikungunya
...
fevers.
...
This
...
species
...
has
...
a
...
large
...
genome
...
with
...
significant
...
population-based
...
size
...
variation.
...
The
...
complete
...
genome
...
sequence
...
was
...
determined
...
for
...
the
...
Foshan
...
strain,
...
an
...
established
...
laboratory
...
colony
...
derived
...
from
...
wild
...
mosquitoes
...
from
...
southeastern
...
China,
...
a
...
region
...
within
...
the
...
historical
...
range
...
of
...
the
...
origin
...
of
...
the
...
species.
...
The
...
genome
...
comprises
...
1,967
...
Mb,
...
the
...
largest
...
mosquito
...
genome
...
sequenced
...
to
...
date,
...
and
...
its
...
size
...
results
...
principally
...
from
...
an
...
abundance
...
of
...
repetitive
...
DNA
...
classes.
...
In
...
addition,
...
expansions
...
of
...
the
...
numbers
...
of
...
members
...
in
...
gene
...
families
...
involved
...
in
...
insecticide-resistance
...
mechanisms,
...
diapause,
...
sex
...
determination,
...
immunity,
...
and
...
olfaction
...
also
...
contribute
...
to
...
the
...
larger
...
size.
...
Portions
...
of
...
integrated
...
flavivirus-like
...
genomes
...
support
...
a
...
shared
...
evolutionary
...
history
...
of
...
association
...
of
...
these
...
viruses
...
with
...
their
...
vector.
...
The
...
large
...
genome
...
repertory
...
may
...
contribute
...
to
...
the
...
adaptability
...
and
...
success
...
of
...
Ae.
...
albopictus
...
as
...
an
...
invasive
...
species.
...
<br/>
...
</p><p>PMID:
...
26483478
...
...
...
...
...
...
</p>
...
]]></description>
...
<author>
...
Chen
...
XG,
...
Jiang
...
X,
...
Gu
...
J,
...
Xu
...
M,
...
Wu
...
Y,
...
Deng
...
Y,
...
Zhang
...
C,
...
Bonizzoni
...
M,
...
Dermauw
...
W,
...
Vontas
...
J,
...
Armbruster
...
P,
...
Huang
...
X,
...
Yang
...
Y,
...
Zhang
...
H,
...
He
...
W,
...
Peng
...
H,
...
Liu
...
Y,
...
Wu
...
K,
...
Chen
...
J,
...
Lirakis
...
M,
...
Topalis
...
P,
...
Van
...
Leeuwen
...
T,
...
Hall
...
AB,
...
Jiang
...
X,
...
Thorpe
...
C,
...
Mueller
...
RL,
...
Sun
...
C,
...
Waterhouse
...
RM,
...
Yan
...
G,
...
Tu
...
ZJ,
...
Fang
...
X,
...
James
...
AA</author>
...
<category>Proc
...
Natl
...
Acad
...
Sci
...
U
...
S
...
A</category>
...
<guid
...
isPermaLink="false">PubMed:26483478</guid>
...
</item>
...
<item>
<title>BIOSAFETY.
...
Safeguarding
...
gene
...
drive
...
experiments
...
in
...
the
...
laboratory.</title>
...
<link>https://www.ncbi.nlm.nih.gov/pubmed/26229113?dopt=
...
Abstract</link>
...
<description>
<![CDATA[<table
...
border="0"
...
width="100%"><tr><td
...
align="left"><a
...
href="http://www.sciencemag.org/cgi/pmidlookup?view=long&
...
pmid=26229113"><img
...
src="//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:
...
-highwire.stanford.edu-icons-externalservices-pubmed-sci_full.gif"
...
border="0"/></a>
...
<a
...
href="https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/26229113/"><img
...
src="//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:
...
-www.ncbi.nlm.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc-MS.gif"
...
border="0"/></a>
...
</td><td
...
align="right"><a
...
href="https://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&
...
cmd=Link&
...
LinkName=pubmed_pubmed&
...
from_uid=26229113">Related
...
Articles</a></td></tr><
...
/table>
<p><b>BIOSAFETY.
...
Safeguarding
...
gene
...
drive
...
experiments
...
in
...
the
...
laboratory.</b></p>
...
<p>Science.
...
2015
...
Aug
...
28;349(6251):927-9</p>
...
<p>Authors:
...
Akbari
...
OS,
...
Bellen
...
HJ,
...
Bier
...
E,
...
Bullock
...
SL,
...
Burt
...
A,
...
Church
...
GM,
...
Cook
...
KR,
...
Duchek
...
P,
...
Edwards
...
OR,
...
Esvelt
...
KM,
...
Gantz
...
VM,
...
Golic
...
KG,
...
Gratz
...
SJ,
...
Harrison
...
MM,
...
Hayes
...
KR,
...
James
...
AA,
...
Kaufman
...
TC,
...
Knoblich
...
J,
...
Malik
...
HS,
...
Matthews
...
KA,
...
O'Connor-Giles
...
KM,
...
Parks
...
AL,
...
Perrimon
...
N,
...
Port
...
F,
...
Russell
...
S,
...
Ueda
...
R,
...
Wildonger
...
J</p>
...
<p>PMID:
...
26229113
...
...
...
...
...
...
</p>
...
]]></description>
...
<author>
...
Akbari
...
OS,
...
Bellen
...
HJ,
...
Bier
...
E,
...
Bullock
...
SL,
...
Burt
...
A,
...
Church
...
GM,
...
Cook
...
KR,
...
Duchek
...
P,
...
Edwards
...
OR,
...
Esvelt
...
KM,
...
Gantz
...
VM,
...
Golic
...
KG,
...
Gratz
...
SJ,
...
Harrison
...
MM,
...
Hayes
...
KR,
...
James
...
AA,
...
Kaufman
...
TC,
...
Knoblich
...
J,
...
Malik
...
HS,
...
Matthews
...
KA,
...
O'Connor-Giles
...
KM,
...
Parks
...
AL,
...
Perrimon
...
N,
...
Port
...
F,
...
Russell
...
S,
...
Ueda
...
R,
...
Wildonger
...
J</author>
...
<category>Science</category>
...
<guid
...
isPermaLink="false">PubMed:26229113</guid>
...
</item>
...
<item>
<title>MiR-2
...
family
...
targets
...
awd
...
and
...
fng
...
to
...
regulate
...
wing
...
morphogenesis
...
in
...
Bombyx
...
mori.</title>
...
<link>https://www.ncbi.nlm.nih.gov/pubmed/26037405?dopt=
...
Abstract</link>
...
<description>
<![CDATA[<table
...
border="0"
...
width="100%"><tr><td
...
align="left"><a
...
href="http://www.tandfonline.com/doi/full/10.1080/15476286.2015.1048957"><img
...
src="//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:-
...
www.tandfonline.com-templates-jsp
...
_style2-_tandf-images-tandf100x25.gif"
...
border="0"/></a>
...
<a
...
href="https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/26037405/"><img
...
src="//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:--www.ncbi.nlm.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif"
...
border="0"/></a>
...
</td><td
...
align="right"><a
...
href="https://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&
...
cmd=Link&
...
LinkName=pubmed_pubmed&
...
from_uid=26037405">Related
...
Articles</a></td></tr></table>
...
<p><b>MiR-2
...
family
...
targets
...
awd
...
and
...
fng
...
to
...
regulate
...
wing
...
morphogenesis
...
in
...
Bombyx
...
mori.</b></p>
...
<p>RNA Biol.
...
2015;12(7):742-8</p>
...
<p>Authors:
...
Ling
...
L,
...
Ge
...
X,
...
Li
...
Z,
...
Zeng
...
B,
...
Xu
...
J,
...
Chen
...
X,
...
Shang
...
P,
...
James
...
AA,
...
Huang
...
Y,
...
Tan
...
A</p>
...
<p>Abstract<br/>
...
MicroRNAs (miRNAs)
...
are
...
post-transcriptional
...
regulators
...
that
...
target
...
specific
...
mRNAs
...
for
...
repression
...
and
...
thus
...
play
...
key
...
roles
...
in
...
many
...
biological
...
processes,
...
including
...
insect
...
wing
...
morphogenesis.
...
miR-2
...
is
...
an
...
invertebrate-specific
...
miRNA
...
family
...
that
...
has
...
been
...
predicted
...
in
...
the
...
fruit
...
fly,
...
Drosophila
...
melanogaster,
...
to
...
be
...
involved
...
in
...
regulating
...
the
...
Notch
...
signaling
...
pathway.
...
We
...
show
...
here
...
that
...
miR-2
...
plays
...
a
...
critical
...
role
...
in
...
wing
...
morphogenesis
...
in
...
the
...
silkworm,
...
Bombyx
...
mori,
...
a
...
lepidopteran
...
model
...
insect.
...
Transgenic
...
over-expression
...
of
...
a
...
miR-2
...
cluster
...
using
...
a
...
Gal4/UAS
...
system
...
results
...
in
...
deformed
...
adult
...
wings,
...
supporting
...
the
...
conclusion
...
that
...
miR-2
...
regulates
...
functions
...
essential
...
for
...
normal
...
wing
...
morphogenesis.
...
Two
...
genes,
...
abnormal
...
wing
...
disc
...
(awd)
...
and
...
fringe
...
(fng),
...
which
...
are
...
positive
...
regulators
...
in
...
Notch
...
signaling,
...
are
...
identified
...
as
...
miR-2
...
targets
...
and
...
validated
...
by
...
a
...
dual-luciferase
...
reporter
...
assay.
...
The
...
relative
...
abundance
...
of
...
both
...
awd
...
and
...
fng
...
expression
...
products
...
was
...
reduced
...
significantly
...
in
...
transgenic
...
animals,
...
implicating
...
them
...
in
...
the
...
abnormal
...
wing
...
phenotype.
...
Furthermore,
...
somatic
...
mutagenesis
...
analysis
...
of
...
awd
...
and
...
fng
...
using
...
the
...
CRISPR/Cas9
...
system
...
and
...
knock-out
...
mutants
...
also
...
resulted
...
in
...
deformed
...
wings
...
similar
...
to
...
those
...
observed
...
in
...
the
...
miR-2
...
overexpression
...
transgenic
...
animals.
...
The
...
critical
...
role
...
of
...
miR-2
...
in
...
Bombyx
...
wing
...
morphogenesis
...
may
...
provide
...
a
...
potential
...
target
...
in
...
future
...
lepidopteran
...
pest
...
control.
...
<br/>
...
</p><p>PMID:
...
26037405
...
...
...
...
...
...
</p>
...
]]></description>
...
<author>
...
Ling
...
L,
...
Ge
...
X,
...
Li
...
Z,
...
Zeng
...
B,
...
Xu
...
J,
...
Chen
...
X,
...
Shang
...
P,
...
James
...
AA,
...
Huang
...
Y,
...
Tan
...
A</author>
...
<category>RNA
...
Biol</category>
...
<guid
...
isPermaLink="false">PubMed:26037405</guid>
...
</item>
...
<item>
<title>Protein phosphorylation during Plasmodium berghei gametogenesis.</title>
...
<link>https://www.ncbi.nlm.nih.gov/pubmed/26008612?dopt=
...
Abstract</link>
...
<description>
<![CDATA[<table
...
border="0"
...
width="100%"><tr><td
...
align="left"><a
...
href="https://linkinghub.elsevier.com/retrieve/pii/S0014-4894(15)00146-0"><img
...
src="//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:-
...
linkinghub.elsevier.com-ihub-images-PubMedLink.gif"
...
border="0"/></a>
...
<a
...
href="https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/26008612/"><img
...
src="//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:-
...
www.ncbi.nlm.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc-MS.gif"
...
border="0"/></a>
...
</td><td
...
align="right"><a
...
href="https://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&
...
cmd=Link&
...
LinkName=pubmed_pubmed&
...
from_uid=26008612">Related
...
Articles</a></td></tr></table>
...
<p><b>Protein phosphorylation during Plasmodium berghei gametogenesis.</b></p>
...
<p>Exp Parasitol.
...
2015
...
Sep;156:49-60</p>
...
<p>Authors:
...
Alonso-Morales
...
A,
...
González-López
...
L,
...
Cázares-Raga
...
FE,
...
Cortés-Martínez
...
L,
...
Torres-Monzón
...
JA,
...
Gallegos-Pérez
...
JL,
...
Rodríguez
...
MH,
...
James
...
AA,
...
Hernández-Hernández
...
Fde
...
L</p>
...
<p>Abstract<br/>
...
Plasmodium gametogenesis within the mosquito midgut is a complex differentiation process involving signaling mediated by phosphorylation, which modulate metabolic routes and protein synthesis required to complete this development. However, the mechanisms leading to gametogenesis activation are poorly understood. We analyzed protein phosphorylation during Plasmodium berghei gametogenesis in vitro in serum-free medium using bidimensional electrophoresis (2-DE)
...
combined
...
with
...
immunoblotting
...
(IB)
...
and
...
antibodies
...
specific
...
to
...
phosphorylated
...
serine,
...
threonine
...
and
...
tyrosine.
...
Approximately
...
75
...
protein
...
exhibited
...
phosphorylation
...
changes,
...
of
...
which
...
23
...
were
...
identified
...
by
...
mass
...
spectrometry.
...
These
...
included
...
components
...
of
...
the
...
cytoskeleton,
...
heat
...
shock
...
proteins,
...
and
...
proteins
...
involved
...
in
...
DNA
...
synthesis
...
and
...
signaling
...
pathways
...
among
...
others.
...
Novel
...
phosphorylation
...
events
...
support
...
a
...
role
...
for
...
these
...
proteins
...
during
...
gametogenesis.
...
The
...
phosphorylation
...
sites
...
of
...
six
...
of
...
the
...
identified
...
proteins,
...
HSP70,
...
WD40
...
repeat
...
protein
...
msi1,
...
enolase,
...
actin-1
...
and
...
two
...
isoforms
...
of
...
large
...
subunit
...
of
...
ribonucleoside
...
reductase
...
were
...
investigated
...
using
...
TiO2
...
phosphopeptides
...
enrichment
...
and
...
tandem
...
mass
...
spectrometry.
...
In
...
addition,
...
transient
...
exposure
...
to
...
hydroxyurea,
...
an
...
inhibitor
...
of
...
ribonucleoside
...
reductase,
...
impaired
...
male
...
gametocytes
...
exflagellation
...
in
...
a
...
dose-dependent
...
manner,
...
and
...
provides
...
a
...
resource
...
for
...
functional
...
studies.
...
<br/>
...
</p><p>PMID:
...
26008612
...
...
...
...
...
...
</p>
...
]]></description>
...
<author>
...
Alonso-Morales
...
A,
...
González-López
...
L,
...
Cázares-Raga
...
FE,
...
Cortés-Martínez
...
L,
...
Torres-Monzón
...
JA,
...
Gallegos-Pérez
...
JL,
...
Rodríguez
...
MH,
...
James
...
AA,
...
Hernández-Hernández
...
Fde
...
L</author>
...
<category>Exp
...
Parasitol</category>
...
<guid
...
isPermaLink="false">PubMed:26008612</guid>
...
</item>
...
<item>
<title>Molecular epidemiology of Plasmodium vivax and Plasmodium falciparum malaria among Duffy-positive
...
and
...
Duffy-negative
...
populations
...
in
...
Ethiopia.</title>
...
<link>https://www.ncbi.nlm.nih.gov/pubmed/25884875?dopt=
...
Abstract</link>
...
<description>
<![CDATA[<table
...
border="0"
...
width="100%"><tr><td
...
align="left"><a
...
href="https://malariajournal.biomedcentral.com/articles/10.1186/s12936-015-0596-4"><img
...
src="//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:
...
-www.biomedcentral.com-graphics-pubmed-BioMedCentral_free_1.png"
...
border="0"/></a>
...
<a
...
href="https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25884875/"><img
...
src="//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:-
...
www.ncbi.nlm.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif"
...
border="0"/></a>
...
</td><td
...
align="right"><a
...
href="https://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&
...
cmd=Link&
...
LinkName=pubmed_pubmed&
...
from_uid=25884875">Related
...
Articles</a></td></tr></table>
...
<p><b>Molecular epidemiology of Plasmodium vivax and Plasmodium falciparum malaria among Duffy-positive
...
and
...
Duffy-negative
...
populations
...
in
...
Ethiopia.</b></p>
...
<p>Malar J.
...
2015
...
Feb
...
19;14:84</p>
...
<p>Authors:
...
Lo
...
E,
...
Yewhalaw
...
D,
...
Zhong
...
D,
...
Zemene
...
E,
...
Degefa
...
T,
...
Tushune
...
K,
...
Ha
...
M,
...
Lee
...
MC,
...
James
...
AA,
...
Yan
...
G</p>
...
<p>Abstract<br/>
...
BACKGROUND:
...
Malaria
...
is
...
the
...
most
...
prevalent
...
communicable
...
disease
...
in
...
Ethiopia,
...
with
...
75%
...
of
...
the
...
country's
...
landmass
...
classified
...
as
...
endemic
...
for
...
malaria.
...
Accurate
...
information
...
on
...
the
...
distribution
...
and
...
clinical
...
prevalence
...
of
...
Plasmodium
...
vivax
...
and
...
Plasmodium
...
falciparum
...
malaria
...
in
...
endemic
...
areas,
...
as
...
well
...
as
...
in
...
Duffy-negative
...
populations,
...
is
...
essential
...
to
...
develop
...
integrated
...
control
...
strategies.<br/>
...
METHODS:
...
A
...
total
...
of
...
390
...
and
...
416
...
community
...
and
...
clinical
...
samples,
...
respectively,
...
representing
...
different
...
localities
...
and
...
age
...
groups
...
across
...
Ethiopia
...
were
...
examined.
...
Malaria
...
prevalence
...
was
...
estimated
...
using
...
nested
...
PCR
...
of
...
the
...
18S
...
rRNA
...
region.
...
Parasite
...
gene
...
copy
...
number
...
was
...
measured
...
by
...
quantitative
...
real-time
...
PCR
...
and
...
compared
...
between
...
symptomatic
...
and
...
asymptomatic
...
samples,
...
as
...
well
...
as
...
between
...
children/adolescents
...
and
...
adults
...
from
...
the
...
local
...
community.
...
An
...
approximately
...
500-bp
...
segment
...
of
...
the
...
human
...
DARC
...
gene
...
was
...
amplified
...
and
...
sequenced
...
to
...
identify
...
Duffy
...
genotype
...
at
...
the
...
-33rd
...
nucleotide
...
position
...
for
...
all
...
the
...
clinical
...
and
...
community
...
samples.<br/>
...
RESULTS:
...
Plasmodium
...
vivax
...
prevalence
...
was
...
higher
...
in
...
the
...
south
...
while
...
P.
...
falciparum
...
was
...
higher
...
in
...
the
...
north.
...
The
...
prevalence
...
of
...
P.
...
vivax
...
and
...
P.
...
falciparum
...
malaria
...
is
...
the
...
highest
...
in
...
children
...
compared
...
to
...
adolescents
...
and
...
adults.
...
Four
...
P.
...
vivax
...
infections
...
were
...
detected
...
among
...
the
...
Duffy-negative
...
samples.
...
Samples
...
from
...
asymptomatic
...
individuals
...
show
...
a
...
significantly
...
lower
...
parasite
...
gene
...
copy
...
number
...
than
...
those
...
from
...
symptomatic
...
infections
...
for
...
P.
...
vivax
...
and
...
P.
...
falciparum.<br/>
...
CONCLUSIONS:
...
Geographical
...
and
...
age
...
differences
...
influence
...
the
...
distribution
...
of
...
P.
...
vivax
...
and
...
P.
...
falciparum
...
malaria
...
in
...
Ethiopia.
...
These
...
findings
...
offer
...
evidence-based
...
guidelines
...
in
...
targeting
...
malaria
...
control
...
efforts
...
in
...
the
...
country.<br/>
...
</p><p>PMID:
...
25884875
...
...
...
...
...
...
</p>
...
]]></description>
...
<author>
...
Lo
...
E,
...
Yewhalaw
...
D,
...
Zhong
...
D,
...
Zemene
...
E,
...
Degefa
...
T,
...
Tushune
...
K,
...
Ha
...
M,
...
Lee
...
MC,
...
James
...
AA,
...
Yan
...
G</author>
...
<category>Malar
...
J</category>
...
<guid
...
isPermaLink="false">PubMed:25884875</guid>
...
</item>
...
<item>
<title>Maternal germline-specific
...
genes
...
in
...
the
...
Asian
...
malaria
...
mosquito
...
Anopheles
...
stephensi:
...
characterization
...
and
...
application
...
for
...
disease
...
control.</title>
...
<link>https://www.ncbi.nlm.nih.gov/pubmed/25480960?dopt=
...
Abstract</link>
...
<description>
<![CDATA[<table
...
border="0"
...
width="100%"><tr><td
...
align="left"><a
...
href="http://www.g3journal.org/cgi/pmidlookup?view=long&
...
pmid=25480960"><img
...
src="//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:
...
-highwire.stanford.edu-icons-externalservices-pubmed-ggg.gif"
...
border="0"/></a>
...
<a
...
href="https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25480960/"><img
...
src="//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:
...
-www.ncbi.nlm.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif"
...
border="0"/></a>
...
</td><td
...
align="right"><a
...
href="https://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&
...
cmd=Link&
...
LinkName=pubmed_pubmed&
...
from_uid=25480960">Related
...
Articles</a></td></tr></table>
...
<p><b>Maternal germline-specific
...
genes
...
in
...
the
...
Asian
...
malaria
...
mosquito
...
Anopheles
...
stephensi:
...
characterization
...
and
...
application
...
for
...
disease
...
control.</b></p>
...
<p>G3 (Bethesda).
...
2014
...
Dec
...
05;5(2):157-66</p>
...
<p>Authors:
...
Biedler
...
JK,
...
Qi
...
Y,
...
Pledger
...
D,
...
James
...
AA,
...
Tu
...
Z</p>
...
<p>Abstract<br/>
...
Anopheles stephensi is a principal vector of urban malaria on the Indian subcontinent and an emerging model for molecular and genetic studies of mosquito biology. To enhance our understanding of female mosquito reproduction, and to develop new tools for basic research and for genetic strategies to control mosquito-borne infectious diseases, we identified 79 genes that displayed previtellogenic germline-specific expression based on RNA-Seq data generated from 11 life stage-specific and sex-specific samples. Analysis of this gene set provided insights into the biology and evolution of female reproduction. Promoters from two of these candidates, vitellogenin receptor and nanos, were used in independent transgenic cassettes for the expression of artificial microRNAs against suspected mosquito maternal-effect genes, discontinuous actin hexagon and myd88. We show these promoters have early germline-specific expression and demonstrate 73% and 42% knockdown of myd88 and discontinuous actin hexagon mRNA in ovaries 48 hr after blood meal, respectively. Additionally, we demonstrate maternal-specific delivery of mRNA and protein to progeny embryos. We discuss the application of this system of maternal delivery of mRNA/miRNA/protein in research on mosquito reproduction and embryonic development, and for the development of a gene drive system based on maternal-effect dominant embryonic arrest. <br/>
</p><p>PMID: 25480960 PubMed - indexed for MEDLINE</p>
]]></description>
<author> Biedler JK, Qi Y, Pledger D, James AA, Tu Z</author>
<category>G3 (Bethesda)</category>
<guid isPermaLink="false">PubMed:25480960</guid>
...
</item>
...
<item>
<title>Site-specific,
...
TALENs-mediated
...
transformation
...
of
...
Bombyx
...
mori.</title>
<link>https://www.ncbi.nlm.nih.gov/pubmed/25460511?dopt=
...
Abstract</link>
...
<description>
<![CDATA[<table
...
border="0"
...
width="100%"><tr><td
...
align="left"><a
...
href="https://linkinghub.elsevier.com/retrieve/pii/S0965-1748(14)00169-6"><img
...
src="//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:
...
-linkinghub.elsevier.com-ihub-images-PubMedLink.gif"
...
border="0"/></a>
...
<a
...
href="https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25460511/"><img
...
src="//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:
...
-www.ncbi.nlm.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc-MS.gif"
...
border="0"/></a>
...
</td><td
...
align="right"><a
...
href="https://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&
...
cmd=Link&
...
LinkName=pubmed_pubmed&
...
from_uid=25460511">Related
...
Articles</a></td></tr></table>
...
<p><b>Site-specific,
...
TALENs-mediated
...
transformation
...
of
...
Bombyx
...
mori.</b></p>
...
<p>Insect Biochem Mol Biol. 2014 Dec;55:26-30</p>
...
<p>Authors:
...
Wang
...
Y,
...
Tan
...
A,
...
Xu
...
J,
...
Li
...
Z,
...
Zeng
...
B,
...
Ling
...
L,
...
You
...
L,
...
Chen
...
Y,
...
James
...
AA,
...
Huang
...
Y</p>
...
<p>Abstract<br/>
...
Transposon-based
...
genetic
...
transformation
...
has
...
facilitated
...
insect
...
functional
...
genomics
...
and
...
new
...
strategies
...
of
...
pest
...
management.
...
However,
...
there
...
is
...
a
...
need
...
for
...
alternative,
...
site-specific
...
approaches
...
to
...
overcome
...
limitations
...
of
...
random
...
integration
...
(and
...
associated
...
position-effects)
...
and
...
potential
...
instability
...
of
...
inserted
...
transgenes.
...
Here
...
we
...
describe
...
a
...
transposon-free,
...
site-specific
...
genetic
...
transformation
...
system
...
mediated
...
by
...
transcription
...
activator-like
...
effector
...
nucleases
...
(TALENs)
...
in
...
the
...
silkworm,
...
Bombyx
...
mori,
...
a
...
lepidopteran
...
model
...
insect.
...
We
...
successfully
...
established
...
a
...
site-specific
...
transgenic
...
system
...
with
...
comparable
...
transformation
...
efficiency
...
to
...
transposon-based
...
genetic
...
transformation
...
through
...
microinjection
...
of
...
TALENs
...
mRNA
...
targeting
...
the
...
BmBLOS2
...
locus
...
and
...
a
...
linearizable
...
donor
...
plasmid
...
encoding
...
an
...
expression
...
cassette
...
of
...
the
...
DsRed2
...
red
...
fluorescent
...
protein.
...
This
...
system
...
provides
...
a
...
valuable
...
approach
...
for
...
insect
...
transgenesis
...
and
...
will
...
enable
...
future
...
functional
...
gene
...
analysis
...
and
...
generate
...
novel
...
applications
...
in
...
agricultural
...
and
...
medical
...
insect
...
pest-management
...
technologies.
...
<br/>
...
</p><p>PMID:
...
25460511
...
...
</p>
...
]]></description>
...
<author>
...
Wang
...
Y,
...
Tan
...
A,
...
Xu
...
J,
...
Li
...
Z,
...
Zeng
...
B,
...
Ling
...
L,
...
You
...
L,
...
Chen
...
Y,
...
James
...
AA,
...
Huang
...
Y</author>
...
<category>Insect
...
Biochem
...
Mol
...
Biol</category>
...
<guid
...
isPermaLink="false">PubMed:25460511</guid>
...
</item>
...
</channel>
...
</rss>
...
Photo by Jerry Harb