NEWS and EVENTS
Meeting agenda draft for 05/17/2017
Photo by Jerry Harb
AGENDA
UCI Malaria Initiative meeting May 17, 10:30-3:00pm, 4248 McGaugh Hall
Anthony A. James, James Lab Staff
Ethan Bier
Gregory Lanzaro
John Marshall
Collaborator meeting UCI
., UCSD, UCB
May 17, 2017
Room 4248 McGaugh Hall, UCI
10:30-
XXXX4:00PM (lunch provided)
Items
- Introductions
- Administrative
a. Interactive projects
i. TIGS
ii. UCI MI (BMGF)
iii. DARPA UCR
iv. DARPA Broad/MIT
b. Initiative website(s)
c. Forward copies of regulatory documents as approved
d. Lists of team members and responsibilities by UC
3. Improvements to Plasmodium falciparum effector molecules
a) Mosquito promoters
i. An. stephensi
ii. An. gambiae
iii. Midgut
G12
Peritrophic matrix 1
iv. hemolymph
1. Vitellogenin
b) Single-chain antibodies
i. Existing
1. Pfs 25 4B7
2. CSP 2A10
3. Chitinase 1C3
ii. Synthetic
1. Pfs 25
2. Pfs 47
3. Pfs 230
4. CSP
5. Chitinase
c) Other effectors
i. Mosquito ligands (SM1)
ii. Antimicrobial peptides
iii. Atreptic immunity (FREP1)
3) P. vivax effector molecules
a. Catalog of target molecules
i.a. Mosquito promoters
i. An. stephensi
ii. An. gambiae
III. Midgut
1. G12
2. Peritrophic matrix 1
iv. hemolymph
1.Vitellogenin
b. Single-chain antibodies
i. Existing
1. Pfs 25 4B7
2. CSP 2A10
3. Chitinase 1C3
ii. Synthetic
1. Pfs 25
2. Pfs 47
3. Pfs 230
4. CSP
5. Chitinase
6. HAP2
c. Other effectors
i. Mosquito ligands (SM1)
ii. Antimicrobial peptides
iii. Atreptic immunity (FREP1)
4. P. vivax effector molecules
a. Catalog of target molecules
i. Conserved surface proteins in midgut stages of parasites
4)
Improvements to gene drive machinery
a.Options for sex-specific drive and screening approaches
1.Males only
1.Testes promoter searches
a.B2 tubulin
b.Phf7
c.Snail
d.Topi
e.Others
ii.Females only
1.Female promoter searches
a. Nanos
iii.nanos
Pipeline for analysis
1.RT-PCR
2.In situ hybridization
iv.New gene discovery
1.Dissections
2.RNA-seq
b.Genomic target sites
i.Locus with load (khw)
ii.Neutral locus
iii.Positive (additional effector phenotypes [e.g.FREP1])
5)
Cas9 helper lines
Vasa-Cas9 in attP80.9 fully marked
Removal of marker genes
Dominant, no load visible marker gene candidates
Recessive, no load visible marker genes
yellow
ebony
others?
Options for challenge assays
a.Plasmodium falciparum
i.Existing
1.UCSD
2.NIH
ii.To develop
1.UCSD
2.UCI
b.Plasmodium vivax
i.India
ii.other
6) Cas9 helper lines
7) Modelling priorities
8) Administrative
a. Interactive projects
i. TIGS
ii. UCI MI (BMGF)
iii. DARPA UCR
iv. DARPA Broad/MIT
b. Initiative website(s)
c. Forward copies of regulatory documents as approved
d. Lists of team members and responsibilities by UC
Modelling priorities
Genome projects
Domestic pipeline options
Indian pipeline options
Target species
Anopheles culicifacies
An. stephensi
An. subpictus
An. fluviatilis
An. minimus
An. dirus
An. sundaicus
An. baimaii
Aedes aegypti
Ae. albopictus
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