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Meeting agenda draft for 05/17/2017

AGENDA

UCI Malaria Initiative meeting May 17, 10:30-3:00pm, 4248 McGaugh Hall

Anthony A. James, James Lab Staff

Ethan Bier

Gregory Lanzaro

John Marshall

Summary of Activities

OBJECTIVE 1: Project Administration status

1.1 Recruit Project Personnel                          (In Progress)

1.2. Develop comprehensive Project Plan        (Complete)

1.3. Develop decision-making protocol           (In Progress)

1.4. Develop communication strategy             (In Progress)

1.5. Develop central web portal                      (In progress)

(Agreements in place)

OBJECTIVE 2. Develop and test Anopheles gambiae strains carrying autonomous gene-drive systems linked anti-pathogen effector genes

2.1 Recruit Project Personnel                         (In Progress

OBJECTIVE 3. Evaluate field site(s) for population alteration trials

3.1 Recruit Project Personnel

3.2 Evaluation of potential field sites for selection of four candidate sites

3.3 Evaluation of candidate field sites for selecting final field trial site

3.4. Assess “colonizability” of  An. gambiae from candidate field sites

Collaborator meeting UCI. UCSD, UCB

May 17, 2017

Room 4248 McGaugh Hall, UCI

10:30- 4:00

1)    Introductions

2)    Improvements to Plasmodium falciparum effector molecules

1. Mosquito promoters
   1.                                                              i.      An. stephensi
   2.                                                            ii.      An. gambiae
   3.                                                         iii.      Midgut
      1. G12
      2. Peritrophic matrix 1
   4.                                                          iv.      hemolymph
      1. Vitellogenin
2. Single-chain antibodies
   1.                                                              i.      Existing
      1. Pfs 25 4B7
      2. CSP 2A10
      3. Chitinase 1C3
   2.                                                            ii.      Synthetic
      1. Pfs 25
      2. Pfs 47
      3. Pfs 230
      4. CSP
      5. Chitinase

3)    P. vivax effector molecules

1. Catalog of target molecules
   1.                                                              i.      Conserved surface proteins in midgut stages of parasites

4)    Improvements to gene drive machinery

1. Options for sex-specific drive and screening approaches
   1.                                                              i.      Males only
      1. Testes promoter searches
         1. B2 tubulin
         2. Phf7
         3. Snail
         4. Tumi
         5. Others
   2.                                                            ii.      Females only
      1. Female promoter searches
         1.  nanos
2. Genomic target sites
   1.                                                              i.      Locus with load (kh^w)
   2.                                                            ii.      Neutral locus
   3.                                                         iii.      Positive (additional effector phenotypes [e.g.FREP1])

5)    Options for challenge assays

1. Plasmodium falciparum
   1.                                                              i.      Existing
      1. UCSD
      2. NIH
   2.                                                            ii.      To develop
      1. UCSD
      2. UCI
2. Plasmodium vivax
   1.                                                              i.      India

6)    Modelling priorities

7)    Action items

||Completed||Priority||Locked||CreatedDate||CompletedDate||Assignee||Name||
|F|M|F|1493755796794|          |sentelle|Create UCIMI Team Web Portal|
|F|M|F|1493762381088|          |sentelle|Track Regulatory Approvals|
|F|M|F|1493927333292|          |sentelle|Collaboration Meeting 05/17/2017 McGaugh Hall 3rd floor 4248 Irvine, CA|
|F|M|F|1494430729865|          |sentelle|Hiring Staff|