UNIVERSITY OF CALIFORNIA IRVINE MALARIA INITIATIVE
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Year 3 and 4 updates pending
https://docs.google.com/document/d/15BQwmgxQDq3_eLgJUsg0atkYQ6eNwfj3Nch8jbCp_uk/edit?usp=sharing
(updated link)
Project Plan (downloadable verisonversion)
https://docs.google.com/document/d/1YmVlgwOaz_qegRmvrzZ39E8FgXZWkx_18C7w7RJTKpM/edit
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Analysis of remotely sensing data for 13 potential field sites.
For each of the 13 potential field sites under consideration, the time series (or static values, for topography) of several environmental variables will be extracted from remote sensing observations and from model reanalysis (specifically, NASA's MERRA-2). The analysis will quantify the spatial variability of the environment in potential field site, as well as the intra-annual and inter-annual variability at the spatial resolution of each variable. Using a set of well-defined criteria we will select four sites as candidate field sites for this program. The results will provide objective spatio-temporal selection criteria, and will inform the timing of site visits to facilitate mosquito collection.
Decision point 3.2.2a. Completion Date: 6/1/17, Reporting date: 6/30/17; Review date: Annual meeting. Written report with maps and graphics on the environmental data for each of 13 potential field sites.
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Select, based on set criteria, four candidate sites.
Based on the compiled reports of entomology, epidemiology and environmental data, four candidate sites will be selected. Temporal remote sensing data will be used to determine the timing of site visits to maximize Anopheles collections.
Decision point 3.2.3a. Completion Date: 6/30/17, Reporting date: 6/30/17; Review date: Annual meeting. Four sites and a tentative schedule for site visits will be determined.
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Lanzaro, and Cornel - Starts 5/1/17
Establish mosquito rearing protocols & insectary at UC Davis
Applications for the appropriate live mosquito import permits (e.g. CDC and USDA) will be initiated. Appropriate insectary set up will be prepared prior to USDA inspection. Appropriate mosquito rearing supplies will be ordered.
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Lanzaro, Cornel and Lee - Starts 10/1/17
Assess ease of colonization for An. gambiae for each site.
Gravid females will be collected and placed individually in microtubes with water and filter paper for oviposition. Egg clutches separated by single females will be transported back to UC Davis as checked baggage or via shipping. Egg numbers, hatching rate, emergence rate, sex ratio, mating status in cage (spermatheca dissections), feeding preference (and whether they laid eggs in cage conditions) and adult longevity will be recorded for each strain.
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Lanzaro, Cornel, Marshall and Lee - Starts 11/2/18
Prepare a written evaluation of each of the four candidate sites.
Candidate sites will be evaluated on the basis of the following criteria:
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Lanzaro and James - Starts 2/1/19
Make final decision on the project field site(s)
A team meeting will be organized at UC Davis to present the progress toward site evaluation and make recommendations for a field site to PI (James).
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